Transcriptome profiling of e11.25 mouse pancreas: comparison between Ngn3-/- and Ngn3-/- ; Pdx1::Ngn3ER TM pancreatic epithelium

ABSTRACT: Individual pancreatic buds of crosses between Ngn3+/- and Ngn3+/- ; Pdx1::Ngn3ER TM -IRES-GFP were dissected. Pregnant females were injected intra-peritoneal with 2mg of Tamoxifen at e10.25 (9 am) and dissected at e11.25 (9 to 11 am). Most of the mesenchymal tissue was removed to reduce the tissue complexity of the sample and enrich in epithelial pancreas progenitors. Moreover, at that developmental stage, Pdx1 promoter exhibits strong and homogenous expression levels. Rescuing Ngn3 expression only 24 hours before dissection (12h before activity, due to the time to process tamoxifen into the active compound 4OH tamoxifen) enriches the gene list in direct targets of Ngn3.

INSTRUMENT(S): Affymetrix GeneChip Scanner 3000 7G

ORGANISM(S): Mus musculus

SUBMITTER: Anne Grapin-Botton 

PROVIDER: E-MTAB-3139 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress