Identifying new interactors of NGN3 through IP-MS
Ontology highlight
ABSTRACT: NGN3 is a transcription factor whose transient expression during pancreatic development is vital for the generation of endocrine pancreatic cells, including beta cells. NGN3 stabilisation has been shown to induce exocrine-to-endocrine cell plasticity in the murine pancreas, making it a viable target for therapies aiming to replenish beta cells after immune-mediated destruction in type 1 diabetes patients. Here, we set out to identify new interactors of NGN3 that could play a role in its post-translational regulation. We transfected HEK293A cells with HA-tagged NGN3 and carried out immunoprecipitation of the HA-tag, followed by analysis of co-immunoprecipitated interactors via LC-MS/MS.
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)
SUBMITTER: Teodora Manea
LAB HEAD: Rocio Sancho
PROVIDER: PXD033691 | Pride | 2023-03-27
REPOSITORIES: Pride
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