ABSTRACT: Comparison of mouse ND plus and ND minus pancreas (at different developmental stages), islets, and brain. The platform used in most comparisons is Affymetrix MG_U74A version 2. Affymetrix MOE430A was additionally used for one of the comparisons.
INSTRUMENT(S): Affymetrix Fluidics Station 400, G2500A Hewlett Packard
Project description:Listeria monocytogenes is a food-borne pathogen which causes listeriosis. It is an intracellular parasite invading the epithelial cells where it escapes from the vacuole into the host cytoplasm to replicate, using actin-based motility to move within and between cells. The intracellular life cycle is well documented whereas the time spent in the lumen of the intestine is poorly understood. The aim of this study was to investigate the mechanism by which L. monocytogenes adapts to the environment of the small intestine prior to invasion. Specifically, to determine if the PrfA regulon, that encodes the virulence factors of L. monocytogenes, is switched on by signals within the intestinal lumen. L. monocytogenes were grown under aerobic or microaerobic conditions with glucose or glycerol as carbon source.
Project description:The purpose of this study was to investigate the effects of hydrocortisone on osteosarcoma cells and its molecular mechanism, so as to determine whether they can be new therapeutic agents for osteosarcoma. osteosarcoma cells were cultured in vitro and they were divided into control group and HC group. transcriptome sequencing and bioinformatics analysis were performed to analyze the effect of the drugs.
Project description:Time course study of the mouse infection by comparing the genomic transcriptional patterns of Listeria monocytogenes EGDe grown under laboratory conditions (exponential growth phase) with that of in vivo-grown bacteria (in mouse spleens) over three days of infection. Time course study of the mouse infection by comparing the genomic transcriptional patterns of Listeria monocytogenes EGDe grown under laboratory conditions (exponential growth phase) with that of in vivo-grown bacteria (in mouse spleens) over three days of infection.
Project description:In this study, we generate genomic maps of Mediator, Rad2, Pol II, TBP and TFIIH, by ChIP coupled to next generation sequencing technology (ChIP-seq), in wild type strains from Saccharomyces cerevisiae. A related study involving ChIP-chip analysis of Rad2 occupany is also deposited at ArrayExpress under accession number E-MEXP-3875 ( http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-3875 ).
Project description:In this study, we generate genomic maps of Mediator, Pol II, TBP and TFIIH, by ChIP coupled to next generation sequencing technology (ChIP-seq), in wild type (WT) strains and med17-ts mutants from Saccharomyces cerevisiae. Some of the data, concerning WT strains are also deposited at ArrayExpress under accession number E-MTAB-1595 (http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1595). There are 2 series of experiment: 1- WT (see E-MTAB-1595) and mutants med17-98, med17-444, and med17-670 (this submission) 2- WT and mutant med17-444 (this submission).
Project description:L. monocytogenes is a food-borne pathogen which causes listeriosis. The aim of this study was to investigate the global gene expression changes in L. monocytogenes in response to spent and fresh media, in the wild-type and non-aggregating actA-DC strain.
Project description:Pulmonary fibrosis was induced by a single tail vein injection of Bleomycin hydrogen chloride (100 mg/kg body weight; 1/3 LD50; Nippon Kayaku Co. Ltd., Tokyo) to 6- to 8-wk-old mice. Mice (3-5/endpoint) were sacrificed 7, 15 and 23 days post Bleomycin administration and RNA samples were extracted from sagittal sections of the left lung lobes. Pooled RNA samples, after reverse transcription and labeling, were hybridized to cDNA microarrays, manufactured by RIKEN, containing 21168 gene probes.
Project description:While Candida dubliniensis and Candida albicans are very close related species, the later is a far more successful yeast pathogen. Several explanations have been pointed out such as discrepancies in fitness, morphogenesis, adherence, or stress resistance. In this study, we investigate the transcriptional reshuffling of C. albicans and C. dubliniensis under conditions that highlight the difference of stress resistances between these strains.