LMWF_EC

ABSTRACT: ECFCs and HUVECs were washed and placed overnight in EBM-2 + 2% FCS. The next morning, medium was replaced by EBM-2 + 2% FCS + 10 µg/mL LMWF or by EBM-2 + 2% FCS alone. After 6 hours or 24 hours of treatment, cells were washed with PBS, detached with accutase. After washing and centrifugation steps, the pellet was resuspended in RLT buffer (Qiagen, Venlo, Netherlands) containing beta mercaptoethanol (Sigma, St Louis, MO, USA) and stored at -80°C. Total RNA was isolated by using the RNeasy Mini Kit (Qiagen) as recommended by the manufacturer. Total RNA concentration and integrity was assessed by Nanodrop (Thermo Fisher Scientific, Waltham, USA) and by microchip electrophoresis (Agilent technologies, Santa Clara, CA, USA). Total RNA was amplified using a kit from Ambion (Carlsbad, CA, USA) a

ORGANISM(S): Homo Sapiens

SUBMITTER: claire bouvard 

PROVIDER: E-MTAB-3925 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress