Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of mouse primary colonic stem cells, stem cells treated with butyrate, and differentiated colonocytes in vitro

ABSTRACT: Colonic epithelial stem cells were isolated from mouse colons as described in our previous publication (Miyoshi and Stappenbeck. In vitro expansion and genetic modification of gastrointestinal stem cells in spheroid culture. Nature Protocols. 2013. PMID: 24232249) and then grown in 50% L-WRN conditioned medium (for stem cells) and treated with NaCl, 1mM or 10mM butyrate. Other samples of stem cells were treated with differentiation medium to produce colonocytes. Transcription profiling was performed to gain insight into the characteristics distinguishing these treatment and differentiation states.

ORGANISM(S): Mus musculus

SUBMITTER: Gerard Kaiko

PROVIDER: E-MTAB-4005 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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