Total and nuclear RNA-seq of whole-embryo and mesodermal cells
Ontology highlight
ABSTRACT: Whole-transcriptome analysis using total RNA-seq (rRNA depleted) of Drosophila melanogaster samples from whole-embryo or mesodermal FACS-sorted cells (using a transgenic fly line expressing a EGFP-tagged protein in mesoderm, during different stages of early embryonic development.
Project description:5'Cap-Analysis of Gene Expression (5' CAGE) from mesodermal and whole embryo RNA at three different time intervals during development.
Project description:5'Cap-Analysis of Gene Expression (5' CAGE) from mesodermal and whole embryo RNA at three different time intervals during development.
Project description:The aim of the experiment was to analyse the modification of the change in expression of genes encoding enzymes involved in the modification of cell wall polysaccharide structure during development.
Project description:After 7 days under normal culture conditions, an iron stress was imposed by addition of iron citrate (500 uM final concentration). This time constituted the time zero (T0) of the time-course experiment.<br> Cells were collected from separate Erlenmeyer flasks at times T0, T+5 min, T+15 min, T+30 min, T+60 min, T+6 h, and T+24h.<br> After filtration, cells were washed with water twice, frozen in liquid nitrogen and stored at 80C before processing for RNA extraction.
Project description:TDNA insertion mutants in CYP98A3 were produced by K. Feldmann, and studied through transcriptomic analysis against the wild type WS (specific insertion was checked by PCR and segregation analysis). Characterization of knockout mutants for P450s can be very helpful to fully understand the function of the disrupted gene, and is expected to generate information about the molecular basis of mutants phenotype.
Project description:A functional study of genes that might play a role in DNA repair/recombination and in the response to oxidative stress (Microarrays expression studies, Microbiological assays, In planta functional studies). Which Arabidopsis thaliana genes are induced by ionising radiations? Comparison of SADE transcriptome data (Af1999083) and micro-arrays transcriptome data.
Project description:which Arabidopsis thaliana genes are induced by ionising radiations? time course: 0.75h, 1.5h, 3h, 5h <br>dose:gamma rays irradiated vs non irradiated 4 days old seedlings
Project description:Seedlings were given a dose of 100 Gray with a 60 cobalt source at a dose rate of 22 gray min -1<br> comparison irradiated Wild Type vs non irradiated Wild Type<br> comparison irradiated mutant ATM vs non irradiated mutant ATM
Project description:Crosslinked chromatin was digested with Micrococcal nuclease and immunopurified for Rpb3, a subunit of RNA Polyermase II. chIP'd DNA was hybridized to Drosophila tiling arrays and nucleosomes that were bound by Pol II were mapped.