Alternative splicing landscape in Arabidopsis

ABSTRACT: Alternative splicing (AS) is a key regulatory mechanism that contributes to transcriptome and proteome diversity. As very few genome-wide studies analyzing AS in plants are available, we have performed high-throughput sequencing of a normalized cDNA library which resulted in a high coverage transcriptome map of Arabidopsis. We detect ~150,000 splice junctions derived mostly from typical plant introns, including a large number of U12 introns (2,069). Around 61% of multi-exonic genes are alternatively spliced under normal growth conditions. These values are much higher than previously reported and imply a significant role of AS in Arabidopsis transcriptome complexity. Moreover we provide experimental validation of 540 AS transcripts (from 256 genes coding for important regulatory factors) us

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Maria Kalyna 

PROVIDER: E-MTAB-4288 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress