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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Arabidopsis thaliana strain:Col-0 Transcriptome or Gene expression

ABSTRACT: We evaluated differential RNA-seq coverage of all TAIR10-annotated introns in Arabidopsis seedlings subjected to heat stress. Transcriptome analyses of plants infected with bacteria suggested the untreated upf1-5 mutant was enriched not only with pathogen defense-associated mRNAs but also with transcripts encoding genes involved in the general abiotic stress responses. Therefore, we reasoned that global IR events in the upf1-5 mutant and in environmentally stressed wild-type plants may show similarities. Indeed, the transcriptomes of upf1-5 mutant and the heat-stressed wild-type seedlings shared an overlapping set of differentially expressed introns

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Sergei Filichkin

PROVIDER: E-MTAB-4292 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Environmental stresses modulate abundance and timing of alternatively spliced circadian transcripts in Arabidopsis.

Filichkin Sergei A SA Cumbie Jason S JS Dharmawardhana Palitha P Jaiswal Pankaj P Chang Jeff H JH Palusa Saiprasad G SG Reddy A S N AS Megraw Molly M Mockler Todd C TC

Molecular plant 20150108 2

Environmental stresses profoundly altered accumulation of nonsense mRNAs including intron-retaining (IR) transcripts in Arabidopsis. Temporal patterns of stress-induced IR mRNAs were dissected using both oscillating and non-oscillating transcripts. Broad-range thermal cycles triggered a sharp increase in the long IR CCA1 isoforms and altered their phasing to different times of day. Both abiotic and biotic stresses such as drought or Pseudomonas syringae infection induced a similar increase. Ther ...[more]

PMID: 25680774

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