Identification of down stream genes in heat treated HSF triple mutants (hsfA1a, hsfA1b, and hsfA1d)
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ABSTRACT: To characterize plant heat shock-responsive genes and to identify identified genes that are regulated by of HSFs underheat shock conditions, transcriptome analysis of rice was conducted using microarray. Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07â1.08) under a 16 h light/8 h dark photoperiod (50 ± 10 μmol photons mâ2 sâ1) at 22°C, and were treated for 30 min at 37°C.
Project description:To understand the Abscisic Acid (ABA) signaling in response to dehydration stress, we performed analysis of gene expression using Arabidopsis wild-type plants and the nced3-2 mutant under dehydration stress. The nced3-2 mutant is an Arabidopsis T-DNA tagged knock-out mutant of the NCED3 gene, which has an essential role in dehydration-inducible ABA biosynthesis. Arabidopsis plants were grown in in soil (verdenite 40 mmΦ, Verde Co., Ltd., Kanagawa, Japan) in a cell strainer (Falcon, 40 μm; Corning Inc., NY, USA). Plants were grown at 22°C for 3 weeks under illumination (40â60 μmol m-2s-1; 16 h light/8 h darkness). Three-week-old plants were exposed to dehydration stress by being denied water for 6, 24, 48, or 72 h.
Project description:To characterize plant heat stress-responsive genes and to clarify the heat stress-responsive transcription pathways, the transcriptome analysis of rice was conducted using microarray. Rice (Oryza sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks with a 12 h light (28°C)/12 h dark (25°C) regimen (ca. 1500 μmol photons mâ2 sâ1) and were treated for 30 min at 42°C.
Project description:To characterize plant HS-responsive genes, the transcriptome analysis of rice was conducted using microarray. Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07â1.08) under a 16 h light/8 h dark photoperiod (50 ± 10 μmol photons mâ2 sâ1) at 22°C, and were treated for 30 min at 37°C.
Project description:To characterize heat stress-responsive genes and to clarify the heat stress-responsive transcription pathways, transcriptome analysis of soybean was conducted using microarray. Soybean (Glycine max cv. Williams82) were grown in plastic pots filled with nutrient soil for 2 weeks with a 12 h light (28°C)/12 h dark (25°C) regimen (ca. 1500 μmol photons mâ2 sâ1) and were treated for 30 min at 42°C.
Project description:To characterize plant heat stress-responsive genes and to clarify the heat stress-responsive transcription pathways, the transcriptome analysis of maize was conducted using microarray. Maize (Zea mays cv. B73) were grown in plastic pots filled with nutrient soil for 2 weeks with a 12 h light (28°C)/12 h dark (25°C) regimen (ca. 1500 μmol photons mâ2 sâ1) and were treated for 30 min at 42°C.
Project description:To characterize plant heat stress-responsive genes and to clarify the heat stress-responsive transcription pathways, the transcriptome analysis of rice was conducted using microarray. Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07â1.08) under a 16 h light/8 h dark photoperiod (50 ± 10 μmol photons mâ2 sâ1) at 22°C, and were treated for 30 min at 37°C.
Project description:Goal of the study is to characterize the function of OsTB1 in rice. we generated mutant lines for OsTB1 gene. We harvested the basal node containing axillary buds from the mutants as well as those of the corresponding controls. We compared the gene expression of the mutants and the controls to estimate the downstream gene regulated by the OsTB1 gene.