Microarray Analysis of Injured Sciatic Nerves of WT and V30M Mice (Agilent Mouse Whole Genome 4x44k v2)

ABSTRACT: Familial amyloidotic polyneuropathy (FAP) is an autosomal dominant hereditary disorder characterized by the extracellular deposition of mutant transthyretin (TTR). The most common TTR mutation in FAP results from an exchange of a methionine for a valine at position 30 – TTR V30M. In an attempt to establish an animal model of TTR Met-30-associated homozygous familial amyloidotic polyneuropathy and to study the structural and functional properties of human TTR Met 30, it was generated a mouse line carrying a null mutation at the endogenous ttr locus (ttr-/-) and the human mutant ttr gene (6.0-hMet 30) as a transgene. For microarray analysis, messenger RNA (mRNA) from WT and V30M injured sciatic nerves was extracted 7 days post-lesion (n=2 per strain), using the Lipid Tissue Mini Kit (Qia

INSTRUMENT(S): Agilent G2565AB microarray scanner

ORGANISM(S): Mus musculus

SUBMITTER: Diana Raquel Fernandes Martins 

PROVIDER: E-MTAB-4525 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress