Nucleosome eviction in mitosis assists condensin loading and chromosome condensation

ABSTRACT: The experiment was performed to assess the importance of nucleosome eviction for the binding of condensin to chromatin during mitosis. The condensin complex associates with chromatin during mitosis to ensure chromosome condensation and accurate segregation, but how this binding is achieved remains poorly understood. Our study indicates that transcription co-activators Gcn5 and Mst2 assist condensin binding during mitosis by evicting nucleosomes. To reach this conclusion, we mapped nucleosomes, during mitosis, in wild-type and gcn5mst2 mutant strains. This experiment allowed us (1) to identify nucleosome-depleted regions (ndrs) during mitosis and to show that condensin tends to colocalize with ndr at the 3ends of genes, and (2) to confirm the importance of nucleosome eviction from ndrs by

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Schizosaccharomyces pombe 972h-

SUBMITTER: Pascal Bernard 

PROVIDER: E-MTAB-4620 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress