Transcriptional profiling of wild-type parent MC58 strain and ptsH knock-out mutant in Neisseria meningitidis

ABSTRACT: In order to assess global differences in gene expression that may be regulated directly or indirectly by HPr , a global transcriptomic profile analysis of the ΔptsH mutant and the wild-type MC58 strain was performed. cDNA probes were prepared from RNA pools (5 μg) obtained from MC58 wild-type and ΔptsH mutant cells grown in rich GCB broth until mid-logarithmic phase. RNA was extracted from mid-log phase and differential gene expression through microarray analysis was performed. Equal amounts of Cy5- and Cy3-labelled cDNAs were hybridized onto the microarray for 17 h at 65°C according to the Agilent protocol. Three hybridizations were performed using cDNA probes from three independent pools, respectively, corresponding to RNA extracted from a total of nine independent bacterial cultures

ORGANISM(S): Neisseria meningitidis MC58

SUBMITTER: Isabel Delany 

PROVIDER: E-MTAB-4997 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress