Expression profiling of human tissues from obese patients
Ontology highlight
ABSTRACT: This experiment captures the expression profiling in obese with type 2 diabetes and non-diabetic patients, in relevant tissues from the disease: liver, subcutaneous and visceral adipose tissues, and whole blood. Samples were obtained during bariatric surgery and preserved in RNA later at -70 C until the nucleic acid extraction.
Project description:This experiment captures the DNA methylation in obese patients with type 2 diabetes in relevant tissues from the disease: liver, subcutaneous and visceral adipose tissues, and whole blood. Samples were obtained during bariatric surgery and preserved in RNAlater at -70 C in RNAlater, until the nucleic acid extraction.
Project description:Brg1 has been reported to act as a trans-activator for the Wnt pathway by interacting with beta-catenin. Given this interaction and the crucial role Wnt signalling plays in the intestinal homeostasis, we aimed to investigate the effect of Brg1 loss on gene expression in normal and Wnt activated small intestinal epithelium. We used VillinCreERT2 Cre recombinase and loxP targeted allels of Brg1 and Apc to generate 4 cohorts of conditional knock-out mice: Cre-negative controls (n=4), Brg1 deficient (n=4), Apc deficient (n=3) and double Brg1-Apc deficient (n=4). All mice were induced by 4x80mg/kg daily injections of Tamoxifen. Epithelium enriched (gut scrapes) samples of small intestine (jejunum) were collected at day 4 post induction. Loss of Brg1 expression in the small intestinal epithelium at this time point was confirmed by immunohistochemistry.
Project description:Three independent B cell samples infected with either EBV M81 devoid of EBER1+2 or its revertant were subjected to a RNA microarray analysis. RNA was isolated from each sample with the TRIzol reagent and treated with DNase to remove any traces of genomic DNA. Samples were analyzed with the Illumina HT12 platform.
Project description:Our goal was to identify the genes, which are modulated following conditional BSP knockdown for 3 and 6 days. Thus, to elucidate the BSP relevance and to broaden the knowledge on the affected signalling pathways. Cell pellets were collected after 3 and 6 days of cultivating the cells in media with or without doxycycline. There were duplicated total RNA samples for each time interval and each condition.The mRNA expression of the in vitro miRNA treated cells was compared to the respective +dox control.
Project description:Analysis of the effect of CLL development on differentation and gene expression of splenic monocytes. C57BL/6 mice were transplanted with murine CLL cells from Eµ-TCL1 mice and after 6 weeks total RNA was isolated from splenic monocytes from leukemic mice and matched WT controls.
Project description:Evaluate differences in gene expression levels between offspring born after maternal bariatric surgery and their siblings born before surgery Offspring born after maternal bariatric surgery (AMS, N=23) vs. offspring born before maternal surgery (BMS, N=23)
Project description:Alteration of gene expression profile due to Lmna knockout was studied in MEF. Total RNA obtained from Lmna +/+ MEF and Lmna -/- MEF were compared.
Project description:It has been shown that in a mouse model overexpressing spermidine/spermine N1-acetyltransferase (SSAT) in whole body the mice have improved glucose homeostasis, reduced white adipose tissue (WAT) mass and high basal metabolic rate. In this study we investigated the glucose and energy metabolism in another SSAT overexpressing mouse model (MT-SSAT) and in addition created a novel mouse model that has adipose tissue specific SSAT overexpression (aP2-SSAT) in order to elaborate the role of enhanced polyamine catabolism in WAT. In this experiment, gene expression profiles of WAT from wild type, SSAT, MT-SSAT and aP2-SSAT mice were studied.
Project description:A distinct highly invasive subpopulation was identified in breast cancer cell lines. The molecular characteristics of these cells was investigated, revealing a set of genes whose high expression confers the ability to invade. Total RNA isolated from the invasive subpopulation from 2 cells lines was compared to total RNA isolated from 2 noninvasive populations.
Project description:Through analysis of a high-content screen we discovered that mir-203 can impair cell migration by suppressing p63 expression. We further determined that p63 promotes motility by inducing the expression of multiple target genes. Total RNA isolated from cell lines with different rated of motility was compared.