Gene expression patterns in 23 cervical samples of women having gone through different types of child birth

ABSTRACT: We performed a microarray experiment to test the hypothesis that pre-labour premature rupture of fetal membranes (PPROM) has a different underlying pathology from spontaneous premature labour with intact membranes (PTL). We thought that the different genetic signature would be detected in the cervical tissue. Our study included cervical biopsies obtained as previously described from women undergoing caesarean section (CS) following PPROM, PTL, term labour (TL) or delivering at term prior to the presentation of labour (Term no labour –TNL). After quality control of the expression arrays, any association between sample covariates was evaluated and no evidence was found of unexpected associations. The differences in expression levels for each statistical comparison were then computed. We identified genes that were differentially expressed with an adjusted pvalue < 0.01 from hypothesis testing together with a fold change >= 2. The strongest effect, i.e. the contrast with the highest number of DEGs, was observed for “Term labour vs Term not labour” (1,285 genes) while the contrast with the fewest DEGs was “Term labour vs Preterm premature rupture of membranes” (16 genes). A table showing the number of differentially expressed genes, for each comparison, can be found in the Results section of this report. We then examined the number of genes overlapping between the statistical comparisons performed (see section 2.5 of the Results) and found that the contrast “Term labour vs Term not labour” had a large number of DEGs in common with the five other contrasts.

ORGANISM(S): Homo sapiens

SUBMITTER: Sofia Makieva 

PROVIDER: E-MTAB-5354 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress