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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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EPO-regulated targets in E1, E2, and E3 erythroid progenitors

ABSTRACT: Profiling of bone marrow-derived erythroid progenitor cells at E1 (CFU-e), E2 (proerythroblasts), and E3 (maturing erythroblast) stages of development under stress erythropoiesis conditions and in response to EPO challenge. MACS-isolated E1, E2, and E3 stage EPCs were cultured in SP34ex media for 6 hrs in the absence of hematopoietic growth factors and the presence of insulin (to enforce survival and anti-apoptotic effects) and then exposed to rhEPO for 90 minutes. Gene expression analysis was performed using Affymetrix Mouse Genome 430 2.0 arrays; microarray data were analyzed using Bioconductor for R.

ORGANISM(S): Mus musculus

SUBMITTER: Don M. Wojchowski

PROVIDER: E-MTAB-5373 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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