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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Role of CCR2+ myeloid cells in pancreatic Î² cell growth and regeneration

ABSTRACT: Pancreas and spleen were microdissected from wild type (C57B6/SV129 background) E14.5 embryos and P0 newborn pups. Pools of 30-60 tissues were used. Tissues were digested for 1 hour at 37Â°C in HBSS/0.1% collagenase A/20 Âµg/ml DNase I, followed by dissociation of the cell clusters into single cells using a non-enzymatic dissociation medium (Sigma). Single cell suspensions were blocked with mouse IgGs and anti-CD16/32 Abs and immunostained with biotin-anti-CD11b (Biolegend) and RPE-conjugated goat anti-CCR2 (R&D Systems), followed by Cy5.5 conjugated streptavidin. CD11b+CCR2+ cell were then isolated by FACS sorting. mRNA from purified cells was prepared using the RNAeasy kit (Qiagen) and run on a MouseWG-6 v2.0 Expression BeadChip.

ORGANISM(S): Mus musculus

SUBMITTER: Willian da Silveira

PROVIDER: E-MTAB-5427 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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