Gene expression analysis in mouse embryonic stem cells containing β-catenin lysine 49 to alanine mutation

ABSTRACT: β-Catenin is the major co-regulator of the Wnt signalling pathway. β-Catenin lysine 49 is post-translationally modified. The histone methyl transferase Ezh2 trimethylates β-catenin at lysine 49 and the acetyl transferase Cbp acetylates β-catenin at the same lysine. To determine the effects on gene expression embryonic stem cells containing either a Gfp tagged β-catenin wildtype (wt) or lysine 49 to alanine (K49A) loss of function mutation were analysed by micro array expression profiling. To determine the effects on gene expression in the pluripotent state two biological replicates of Gfp β-catenin wt and Gfp β-catenin K49A were analysed. The results showed that genes which affect pluripotency as well as differentiation were altered by β-catenin K49A mutation. To analyse the effe

ORGANISM(S): Mus musculus

SUBMITTER: Katrin Hoffmeyer 

PROVIDER: E-MTAB-5523 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress