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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Microarray of BAC transgenic Zfhx2 p.R1907K mutant mice (4 genomic copies) versus wild-types

ABSTRACT: A novel missense mutation has been identified in a human pain-insensitive family in the gene ZFHX2. We modelled this mutation in mice by changing the orthologous amino acid and carried out pain behaviour tests in mutant BAC transgenic mice. These showed a phenotype of hyposensitivity to noxious thermal stimuli. ZFHX2 has enriched expression in dorsal root ganglia neurons, and so we carried out microarray analyses in mutant vs wild type controls from lumbar DRG to see which genes were deregulated by the mutant ZFHX2 protein. To understand which genes are differentially expressed in lumbar DRG (L1-6) between Zfhx2 p.R1907K BAC transgenic mice (n=5, genomic BAC copy number of 4) and wild-type controls (n=7). Complementary ChIP-seq data for this study have also been deposited at ArrayExpress under accession number E-MTAB-5651 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5651 ).

ORGANISM(S): Mus musculus

SUBMITTER: James Cox

PROVIDER: E-MTAB-5650 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ChIP-seq experiment to determine the genomic binding sites of mutant V5-tagged ZFHX2 R1913K protein in a stable-expressing human SH-SY5Y neuroblastoma cell line

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