RNA-microarray analysis of PLB-985 cells cultured with or without interferon-gamma under both conditions that induce or do not induce cell differentiation

ABSTRACT: Interferon-gamma (IFN-gamma) is approved as a drug to treat chronic granulomatous disease and osteopetrosis and is also used in hyperimmunoglobulin E syndromes. These primary immunodeficiencies involve defects in neutrophils/polymorphonuclear cells. Neutrophils mature in the bone marrow and then enter the blood where they quickly undergo apoptotic cell death with a half-life of 5-10 hours. Therefore we reasoned that IFN-gamma might exert its effects on neutrophils via prolonged exposure to cells undergoing maturation toward a terminally differentiated state in the marrow rather than by its brief exposure to short-lived circulating cells. To explore this possibility we made use of PLB-985 cells which are a myeloblast-like myeloid cell line that can be differentiated into a mature, neutr

INSTRUMENT(S): GeneChip Fluidics Station 450, GeneChip Scanner 3000

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Ellison 

PROVIDER: E-MTAB-5690 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress