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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Role of RccR in the transcriptional control of primary carbon metabolism in Pseudomonas

ABSTRACT: To characterise the RccR regulon, we performed a ChIP-seq assay using a polyclonal RccR antiserum on SBW25 WT/rccR strains grown in minimal pyruvate and glycerol media. We were able to identify 8 RccR binding sites from this experiment including one in the rccR promoter region itself. The peaks identified in our RccR ChIP-seq assays corresponded to strongly enriched regions relative to the respective rccR controls. All 8 peaks were localized in intergenic regions, upstream of one or more genes.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Pseudomonas fluorescens

SUBMITTER: Rosaria Campilongo

PROVIDER: E-MTAB-5745 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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One ligand, two regulators and three binding sites: How KDPG controls primary carbon metabolism in Pseudomonas.

Campilongo Rosaria R Fung Rowena K Y RKY Little Richard H RH Grenga Lucia L Trampari Eleftheria E Pepe Simona S Chandra Govind G Stevenson Clare E M CEM Roncarati Davide D Malone Jacob G JG

PLoS genetics 20170628 6

Effective regulation of primary carbon metabolism is critically important for bacteria to successfully adapt to different environments. We have identified an uncharacterised transcriptional regulator; RccR, that controls this process in response to carbon source availability. Disruption of rccR in the plant-associated microbe Pseudomonas fluorescens inhibits growth in defined media, and compromises its ability to colonise the wheat rhizosphere. Structurally, RccR is almost identical to the Entne ...[more]

PMID: 28658302

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