Role of Fnr1 and Fnr3 in transcriptional regulation under oxygen limitation in Herbaspirillum seropedicae

ABSTRACT: To determine the direct promoter targets of Fnr proteins, we correlated transcriptional changes observed in single fnr1 and fnr3 mutants (E-MTAB-5741) with ChIP-Seq analysis, taking advantage of C-terminally 3xFlag fnr alleles engineered into the H. seropedicae genome. ChIP-seq data were obtained from cultures grown under limited oxygen availability. Using this approach, DNA-binding targets for the H. seropedicae Fnr1 and Fnr3 proteins were unambiguously revealed and correlated with transcript profiles to determine the specific regulons of each protein.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Herbaspirillum seropedicae

SUBMITTER: Marcelo Batista 

PROVIDER: E-MTAB-6395 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress