RNA-seq of muscle from pigs divergent in feed efficiency and product quality
Ontology highlight
ABSTRACT: Feed efficiency (FE) is an indicator of efficiency in converting energy from feed into a tissue that is of major environmental and economic significance.This study was to profile the porcine Longissimus thoracis et lumborum (LTL) muscle transcriptome, examine the product quality from pigs divergent in FE and investigate the functional networks underpinning the potential relationship between product quality and FE.
Project description:Feed efficiency (FE) is an indicator of efficiency in converting energy, attained from macronutrient ingestion, into tissue. Adipose tissue, besides being a master regulator of systemic lipid storage, is also an active endocrine organ that communicates with skeletal muscle, liver and brain to influence appetite, lipid & glucose metabolism and energy homeostasis. Adipose tissue is hypothesised to play a vital part in regulation of FE. The objective of the present study was to sequence the subcutaneous adipose tissue transcriptome in FE-divergent pigs (n=16) and identify relevant biological processes underpinning observed differences in FE.
Project description:Liver is a metabolically complex organ that influences nutrient partitioning and potentially modulates the efficiency of converting energy acquired from macronutrients ingestion into a muscle and/or adipose tissue (referred to as feed efficiency, FE). The objective of this study was to sequence the hepatic tissue transcriptome of pigs divergent for FE and identify relevant biological processes that underpin the differences in liver phenotypes and FE.
Project description:Feed accounts for more than 60% of the costs for pig production, and FE can be measured as residual feed intake (RFI). In this study, we compared the transcriptome difference of longissimus dorsi muscles of Yorkshire pigs with high RFI (RFI_H) and low RFI (RFI_L) using Solexa mRNA sequencing.
Project description:The aim of this experiment was to identify changes in gene expression in muscle and liver between pigs that were divergent in feed efficiency. The animals were selected from two different farms of origin. 48 animals were utilized in this study. Following slaughter RNA Total RNA was extracted from liver and muscle ((25 mg) tissue using Trizol Reagent (Sigma-Aldrich, Arklow, Ireland) according to the manufacturer’s instructions, the crude RNA extract was further purified using the GenElute Mammalian Total RNA Miniprep Kit (RTN70, Sigma-Aldrich). Library construction and sequencing was performed by the next generation sequencing facility at the Institute of Molecular Medicine, University of Leeds, United Kingdom. The RNA-seq libraries were constructed using the Illumina TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA) per the manufacturer’s instructions. One hundred base paired-end sequencing was run on an Illumina HiSeq2000 platform with each pool ran on two lanes on a flow cell.
Project description:Feed accounts for more than 60% of the costs for pig production, and FE can be measured as residual feed intake (RFI). In this study, we compared the transcriptome difference of longissimus dorsi muscles of Yorkshire pigs with high RFI (RFI_H) and low RFI (RFI_L) using Solexa miRNA sequencing.
Project description:This research investigates the influence of nutritional protein restriction (NPR) during prepuberty on FE and the milk transcriptome of dairy Assaf ewes during their first lactation. Additionally, it evaluates the differences in the milk transcriptome between lactating ewes with divergent FE using the feed conversion ratio (FCR) and residual feed intake (RFI) indices and assesses milk gene expression as a predictor of FE.
Project description:Global gene expression profiles of peripheral blood of 35 to 42 day-old Yorkshire pigs with extremely low (more efficient) and high RFI (less efficient) values from two Iowa State University lines (the low RFI line, n = 15, and the high RFI line, n =16) that were divergently selected for RFI during the grow-finish phase were determined by Illumina RNA-seq (100 bp, paired-end) on the Hiseq2000 platform to explore the transcription biomarkers for feed efficiency in pigs.
Project description:Chickens (Cobb500FF) divergent in residual feed intake were analyzed for muscular (M. pectoralis), duodenal, jejunal and ileal transcriptomic profiles.
Project description:Gene expression determination between breast muscle associated with the phenotypic expression of feed efficiency (FE) in a single male broiler line. Goal was to determine the changes of gene expression by feed efficiency (FE). Two-condition experiment, high feed efficiency vs. low feed efficiency. Biological replicates: breast muscles from 4 high feed efficiency male broiler, breast muscles from 4 low feed efficiency male broiler