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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of Bacillus subtilis (i) sigI-rsgI knock-out strain, (ii) rsgI disrupted strain, and (iii) wt strains during growth at 52°C and 37°C

ABSTRACT: The aim of the experiments was to determine the regulon of the Bacillus subtilis alternative sigma factor SigI. Biological relevance: To expand our knowledge about Bacillus subtilis transcriptional network under unfavorable conditions. Experimental workflow overview: Bacillus subtilis 168 trp+ (BaSysBio) was used as the genetic background. (i) sigI-rsgI knock-out, (ii) rsgI knock-out, and (iii) wr strains were cultured in LB medium to mid-exponential phase at 37°C and 52°C. Total RNA was isolated from 3 ml of the culture. rRNA was depleted from the samples with RiboMinus; subsequently RNA-seq libraries were prepared (Illumina compatible NEXTflex Rapid Directional RNA-Seq Kit, Bioo Scientific) and sequenced at the EMBL GeneCore facility. The experiment was performed in three independent replicates.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168

SUBMITTER: Martin Převorovský

PROVIDER: E-MTAB-6314 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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