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Pericyte induced reprogramming of the bone marrow microenvironment drives stem and cancer cell quiescence

ABSTRACT: This dataset was generated by RNA sequencing of whole tibiae of C57Bl/6 mice. For young mice; tibiae from 6-weeks-old mice were used and for aged mice tibiae from 70-weeks-old mice were used. The control, radiation, and chemotherapy samples were generated using 14-weeks-old mice. For radiation treatment; mice were exposed to sublethal irradiation while for chemotherapy samples mice were treated with carboplatin and tibiae were collected

INSTRUMENT(S): Illumina HiSeq 2500, FACS Aria 3

ORGANISM(S): Mus musculus

SUBMITTER: Amit Singh

PROVIDER: E-MTAB-6872 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Proteomics on sera of mice following local high-dose heart irradiation

Project description:Background and Purpose: Cardiotoxicity is a well-known adverse effect of radiation therapy. Measurable abnormalities in the heart function indicate advanced and often irreversible heart damage. Therefore, early detection of cardiac toxicity is necessary to delay and alleviate the development of the disease. The present study investigated long-term serum proteome alterations following local heart irradiation using a mouse model with the aim to detect biomarkers of radiation-induced cardiac toxicity. Materials and Methods: Serum samples from C57BL/6J mice were collected 20 weeks after local heart irradiation with 8 Gy or 16 Gy X-ray; the controls were sham-irradiated. The samples were analyzed by quantitative proteomics based on data-independent acquisition mass spectrometry. The proteomics data were further investigated using bioinformatics and ELISA. Results: The analysis showed radiation-induced changes in the level of several serum proteins involved in the acute phase response, inflammation and cholesterol metabolism. We found significantly enhanced expression of pro-inflammatory cytokines (TNF-, TGF-, IL-1 and IL-6) in the serum of the irradiated mice. The level of free fatty acids, total cholesterol, low density lipoprotein (LDL) and oxidized LDL was increased whereas that of high density lipoprotein was decreased by irradiation. Conclusions: This study provides information on systemic effects of heart irradiation. It elucidates a radiation fingerprint in the serum that may be used to elucidate adverse cardiac effects after radiation therapy.

2021-09-10 | PXD024446 | Pride

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2016-07-04 | PXD003230 | Pride

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2015-06-17 | E-GEOD-58586 | biostudies-arrayexpress

Mouse kidney cisplatin TAILS - The proteome microenvironment determines the protective effect of preconditioning in cisplatin-induced acute kidney injury

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2018-10-29 | PXD007186 | Pride

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