Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Microarray of four types of mouse immune samples including those of the spleen (SP), bone marrow (BM), lymph node (LN), and peripheral blood mononuclear cell (PBMC) and three tumor tissues including MC38, EL4 and LLC

ABSTRACT: We performed MIcroarray experiments on a total of 12 immune organs, including spleen (SP), bone marrow (BM), lymph node (LN) and Peripheral blood mononuclear cell (PBMC) and three tumor tissues including MC38, EL4 and LLC. 12 mice from four immune organs, including the spleen (SP), bone marrow (BM), lymph nodes (LN), and Peripheral blood mononuclear cell (PBMC), were isolated using routine experimental methods.9 mice were randomly divided into 3 groups and implanted subcutaneously(s.c.) with EL4 (2.0*10^5 /mice), MC-38 (1.0*10^6 /mice), and LLC-JSP-t2 (2.0*10^5 /mice) cells, respectively. All mice were sacrificed and tumor tissues were collected when the tumor volume reached 100 mm 3 . The tissues were ground up with liquid nitrogen and the lysates were stocked using Trizol for subsequent array analysis. RNA isolation and hybridization were performed by Capitalbio Technology Corporation according to the standard Affymetrix protocol .

ORGANISM(S): Mus musculus

SUBMITTER: Ziyi Chen 

PROVIDER: E-MTAB-6948 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Inference of immune cell composition on the expression profiles of mouse tissue.

Chen Ziyi Z   Huang Anfei A   Sun Jiya J   Jiang Taijiao T   Qin F Xiao-Feng FX   Wu Aiping A  

Scientific reports 20170113

Mice are some of the widely used experimental animal models for studying human diseases. Defining the compositions of immune cell populations in various tissues from experimental mouse models is critical to understanding the involvement of immune responses in various physiological and patho-physiological conditions. However, non-lymphoid tissues are normally composed of vast and diverse cellular components, which make it difficult to quantify the relative proportions of immune cell types. Here w  ...[more]

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