Sparse feature selection methods identify unexpected global cellular response to strontium-containing materials

ABSTRACT: Despite the increasing sophistication of biomaterials design and functional characterization studies, little is known regarding cells' global response to biomaterials. Here, we combined nontargeted holistic biological and physical science techniques to evaluate how simple strontium ion incorporation within the well-described biomaterial 45S5 bioactive glass (BG) influences the global response of human mesenchymal stem cells. Our objective analyses of whole gene-expression profiles, confirmed by standard molecular biology techniques, revealed that strontium-substituted BG up-regulated the isoprenoid pathway, suggesting an influence on both sterol metabolite synthesis and protein prenylation processes. This up-regulation was accompanied by increases in cellular and membrane cholesterol and l

ORGANISM(S): Homo sapiens

SUBMITTER: Hélène Autefage 

PROVIDER: E-MTAB-7384 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress