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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Knockdown of laminin alpha 5 chain in colorectal cancer cell line HT-29

ABSTRACT: Laminin-mediated signaling via membrane receptors actively participate in most normal biological process and pathologies. Laminins involve in all step of metastatic cascade including tumor cell proliferation, migration, anoikis resistance, extravasation, epithelial to mesenchymal transition etc. The 5-containing laminins (LAMA5) are one of the three types of laminins predominantly expressed in the epithelial basement membrane in the adult intestine as well as during crypt-villus development. Likewise laminins 511 and 521 (laminin-10 and laminin-11) have been shown as important matrix component to facilitate stem cell survival and to maintain their stem cell properties. Thus, it is assuming that laminins play a role in the formation of premetastatic niches. To investigate the role of 5-containing laminins in colorectal cancer we performed the knockdown of LAMA5 gene expression in HT-29 cell line. Here we present the results of comprehensive gene expression analysis using Affymetrix microarrays in cells transduced by shRNA to LAMA5, control nonsense shRNA, and parental cells as well.

ORGANISM(S): Homo sapiens

SUBMITTER: Diana Maltseva

PROVIDER: E-MTAB-7754 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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