Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Human small intestinal organoids grown with a specific protocol to enrich for Paneth cells were treated with an inhbitor (GSK-LSD1) for the epigenetic modulator LSD1.

ABSTRACT: To assess the role of LSD1 in human intestinal epithelium, human small intestinal organoids were treated with an inhibitor for LSD1 (GSK-LSD1) and compared to untreated organoids. The organoids were grown with specific conditions where Paneth cells are present in the organoids as similar experiments in mice show that Paneth cells disappear upon GSK-LSD1 treatment. Similar to mouse intestinal organoids, Paneth cells dissappear upon GSK-LSD1 treatment. Furthermore, we used these gene set enrichment analysis on these microarray data to show that these human intestinal organoids have a similar phenotype as mice epithelium without LSD1.

ORGANISM(S): Homo sapiens

SUBMITTER: Håvard Lindholm 

PROVIDER: E-MTAB-7863 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Intestinal epithelial homeostasis is maintained by adult intestinal stem cells, which, alongside Paneth cells, appear after birth in the neonatal period. We aimed to identify regulators of neonatal intestinal epithelial development by testing a small library of epigenetic modifier inhibitors in Paneth cell-skewed organoid cultures. We found that lysine-specific demethylase 1A (<i>Kdm1a/Lsd1</i>) is absolutely required for Paneth cell differentiation. <i>Lsd1</i>-deficient crypts, devoid of Panet  ...[more]

Similar Datasets

Transcriptomics RNA-seq of small intestinal organoids treated with an inhibitor for the epigenetic modifier LSD1
2020-11-16 | E-MTAB-9077 | biostudies-arrayexpress
Transcriptomics RNA-seq of mouse small intestinal organoids with an intestinal-epithelial specific deletion of LSD1 compared to wild type
2023-08-02 | E-MTAB-10500 | biostudies-arrayexpress
Transcriptomics Stool-derived 16s rRNA V4 sequencing from inducible intestinal-epithelium-LSD1-cKO mice (Villin-CreERT2+ Lsd1fl/fl (icKO)) against WT (Villin-CreERT2+ Lsd1fl/+ (icKO)) controls (before and 1-6 weeks after tamoxifen treatment)
2023-10-31 | E-MTAB-12626 | biostudies-arrayexpress
Proteomics GFI1B and LSD1 repress myeloid traits during megakaryocyte differentiation
2024-03-28 | PXD050401 | Pride
Transcriptomics KDM6 Inhibition induces DNA Damage Response (DDR) during ESC Differentiation but not during self-renewal.
2016-03-11 | E-GEOD-49886 | biostudies-arrayexpress
Transcriptomics Transcription profiling of pituitary glands from wild type and LSD1 knock-out mice
2007-02-13 | E-TABM-223 | biostudies-arrayexpress
Transcriptomics RNA-seq of crypt cells from mouse small intestinal epithelium with an intestinal-epithelial specific deletion of LSD1 compared to wild type crypts
2020-11-16 | E-MTAB-7862 | biostudies-arrayexpress
Transcriptomics GSK-3A and GSK-3B knockdown in AML cell lines
2012-02-27 | E-GEOD-35200 | biostudies-arrayexpress
Transcriptomics Analysis of Gsk-3-Deficient Embryonic Stem Cells Reveals Large-Scale Alterations in Gene Expression
2011-09-14 | E-GEOD-27395 | biostudies-arrayexpress
Transcriptomics Phosphatidylinositol 3-Kinase (PI3K) Signaling via Glycogen Synthase Kinase-3 (Gsk-3) Regulates DNA Methylation of Imprinted Loci.
2010-11-15 | E-GEOD-20015 | biostudies-arrayexpress