Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

14 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell study of neural stem cells derived from human iPSCs reveal distinct progenitor populations with neurogenic and gliogenic potential (2)

ABSTRACT: Single cell RNA-seq study of induced pluripotent stem cell derived neural stem cells. Analysis of gene expression over cell clusters identified inherent presence of neurogenic progenitors and gliogenic progenitors in established neural stem cells. This study aids to explain heterogeneity of neural stem cell identity and resolves gene expression enrichment in subpopulations of diverse progenitors. Processed and quality controlled data sets used for generating figure 2 in published article. Single cell raw data files for experiments are not available for public download.

ORGANISM(S): Homo sapiens

SUBMITTER: Matti Lam

PROVIDER: E-MTAB-8379 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Single cell study of neural stem cells derived from human iPSCs reveal distinct progenitor populations with neurogenic and gliogenic potential (3)

Project description:Single cell RNA-seq study of induced pluripotent stem cell derived neural stem cells. Analysis of gene expression over cell clusters identified inherent presence of neurogenic progenitors and gliogenic progenitors in established nerual stem cells. This study aids to explain heterogeneity of neural stem cell identity and resolves gene expression enrichment in subpopulations of diverse progenitors. Processed and quality controlled data sets used for generating figure 3 in published article. Single cell raw data files for experiments are not available for public download.

2019-10-24 | E-MTAB-8383 | biostudies-arrayexpress

Single cell experiment with iPS derived neural stem cells and 28 day differentiated neuronal cells from healthy individual and NRXN1-a biallelic deletion individual

Project description:iPS cell derived neural stem cells and differentiated cells from one healthy individual and one indivudal carrying NRXN1-a biallelic deletion investigated with single cell RNA-sequencing. Celltype characterization and comparison revealed variations between stem cell identity and in cell differentiation outcome across the two individuals.

2019-07-20 | E-MTAB-7744 | biostudies-arrayexpress

Single cell study of neural stem cells derived from human iPSCs reveal distinct progenitor populations with neurogenic and gliogenic potential (1)

Project description:Single cell RNA-seq study of induced pluripotent stem cell derived neural stem cells. Analysis of gene expression over cell clusters identified inherent presence of neurogenic progenitors and gliogenic progenitors in established neural stem cells. This study aids to explain heterogeneity of neural stem cell identity and resolves gene expression enrichment in subpopulations of diverse progenitors. Processed and quality controlled data sets used for generating figure 4 in published article. Single cell raw data files for experiments were not made available.

2019-10-24 | E-MTAB-8363 | biostudies-arrayexpress

Notch engagement by Jag1 clusters on the nanoscale suggests a force-independent mode of activation

Project description:The Notch signaling pathway is a cell-cell communication system with fundamental roles in embryonic development and the nervous system, including neural stem cell proliferation and differentiation. To investigate the multivalency effect of ligands on the activation of the Notch receptor, we treated iPSc-derived neuroepithelial stem-like (lt-NES) cells with different Jag1 nanopatterns on DNA origami nanostructures.

2023-11-23 | E-MTAB-12439 | biostudies-arrayexpress

RNA-seq of epiblast from mouse embryos at E4.5, E4.75 and E5.0

Project description:In order to gain a global temporal picture of epiblast potency across implantation, we collected mouse embryos at E4.5, E4.75 and E5.0, and dissected the epiblast for deep-sequencing analysis.

2017-11-24 | E-MTAB-5147 | biostudies-arrayexpress

Single-cell RNA-sequencing of Gli1-positive cells from different compartments of the adult mouse epidermis

Project description:Tomato-traced single cells from the interfollicular epidermis including the touch dome (Gli1-Tomato+/Sca1+) and the hair follicle (Gli1-Tomato+/Sca1-) were FACS-sorted, randomly sequenced and clustered into epidermal (sub-)populations.

2023-12-08 | E-MTAB-6031 | biostudies-arrayexpress

Time-course transcriptome analysis of a human neuronal cell line

Project description:RNA-seq analysis on a human neuronal cell line derived from fetal mesencephalon (LUHMES) from day 0 to day 6.

2021-07-31 | E-MTAB-8908 | biostudies-arrayexpress

ATAC-seq experiment at four time points in HEK293 and hESC cells, assessing the time-resolved response on chromatin accessibility upon Wnt/β-catenin signaling activation

Project description:Wnt/β-catenin signaling is a highly organized biochemical cascade that triggers a gene expression program in the signal-receiving cell. The Wnt/β-catenin -driven transcriptional response is involved in virtually all cellular processes during development, homeostasis, and its deregulation causes human disease. However, outstanding questions remain unanswered. Among these, one regards how the Wnt/β-catenin cascade modulates the chromatin behavior: to date, there exists no comprehensive genome-wide annotation of changing chromatin patterns upon Wnt pathway activation. This is important, as shifts in chromatin patterns might underlie how different cells promote diverging gene expression programs in response to Wnt. To address this question, we characterized how Wnt/β-catenin signaling shapes the genome-wide chromatin accessibility landscape in two human cell types, human embryonic kidney cells 293T (HEK293T) and human embryonic stem cells (hESCs), over time. To this end, we treated HEK293T and hESCs with the GSK3 inhibitor/Wnt activator CHIR99021 (10 mM) for 3 days and assessed chromatin accessibility via ATAC-sequencing 4 hours, 24 hours and 3 days after the onset of the stimulation. We found that hESCs respond to Wnt/β-catenin activation by progressively shaping their chromatin accessibility profile in a manner that is consistent with their gradual acquisition of a mesodermal identity: differentiation genes loci open over time, while pluripotency ones close. We refer to this genomic response as plastic. On the other hand, HEK293T, which are known to be highly responsive to Wnt activation, appear more resistant to a long-term Wnt/β-catenin-driven change in cell identity. In this context, the chromatin displays a temporary opening of relevant regions at 4 hours after stimulation, followed by a re-establishment of its pre-stimulation state: we define this transient response as elastic.

2022-09-30 | E-MTAB-12076 | biostudies-arrayexpress

Time-course transcriptome analysis of a human neuronal cell line with RFX2 KO

Project description:RNA-seq analysis on a human neuronal cell line derived from fetal mesencephalon (LUHMES) wild type and RFX2 knockout from day 0 to day 6.

2024-01-31 | E-MTAB-11546 | biostudies-arrayexpress

Single cell RNA-sequencing of retinal bipolar cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-08-25 | E-GEOD-81905 | biostudies-arrayexpress