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RNAseq of pancreata from KRasG12D/RosaMycER treated with Tamoxifen for different times

ABSTRACT: Experiment designed to study the effect of deregulated myc on pancreatic cancer progression and regression. In the KRasG12D/RosaMycER mouse model KRas and MycER are expressed exclusively in the pancreas, but MycER activity depends on Tamoxifen presence. RNAseq was preformed on pancreata from mice treated with Tamoxifen as follows: 1- Normal food. 2- Tamoxifen food 2 weeks. 3- Tamoxifen food 2 weeks, followed by normal food 1 day. 4- Tamoxifen food 2 weeks, followed by normal food 3 days.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Tania Campos

PROVIDER: E-MTAB-8467 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNAseq of pancreatas from KRasG12D/RosaMycER treated with Tamoxifen for different times. MycOFF short times.

Project description:Experiment designed to study the effect of deregulated myc on pancreatic cancer progression and regression. In the KRasG12D/RosaMycER mouse model KRas and MycER are expressed exclusively in the pancreas, but MycER activity depends on Tamoxifen presence. RNAseq was preformed on pancreatas from mice treated with Tamoxifen as follows: 1-Tamoxifen food 2 weeks. 2- Tamoxifen food 2 weeks, followed by normal food 6 hours day. 3- Tamoxifen food 2 weeks, followed by normal food 12 hours.

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RNAseq of pancreatas from KRasG12D/RosaMycER+/- or KRasG12D/RosaMycER+/+ treated with Tamoxifen for 12h

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Project description:Experiment designed to study the effect of deregulated myc on pancreatic cancer progression and regression. In the KRasG12D/RosaMycER mouse model KRas and MycER are expressed exclusively in the pancreas, but MycER activity depends on Tamoxifen presence. For this experiment a mouse was treated with Tamoxifen during 3 weeks, the pancreas was collected and the epithelial cells were grown in vitro in the presence of 4-OHT (4-Hydroxytamoxifen, active form of Tamoxifen). RNAseq were preformed on the cell line treated as follows: 1- 4-OHT all the time. 2- EtOH 6h. 3- EtOH 12h. 4- EtOH 18h.

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RNA-seq of purified cardiomocytes from adult mice (+/- AAV-CCNT1) 4 hours post MycER activation compared controls.

Project description:Aim: To determine RNA expression levels in heart tissues of mice 4 post Myc activation. Mice were either untreated or systemically infected with a cardiomyocyte specific adeno associated virus (AAV9) at 4 to 6 weeks of age. Cardiomyocytes were isolated from adult mice that were either untreated or infected with AAV9-CCNT1 and were either Myh6-cre;R26CAG-c-MycERT2/+ or R26CAG-c-MycERT2/+ following 4 hours of MycER activation via administration of (Z)-4-hydroxytamoxifen.

2020-02-24 | E-MTAB-8515 | biostudies-arrayexpress

RNAseq of pancreata from KRasG12D/RosaMycER treated with Tamoxifen for different times

Project description:RNAseq of pancreata from KRasG12D/RosaMycER treated with Tamoxifen for different times

| PRJEB35067 | ENA

E-MTAB-7595

Project description:RNA-seq of heart tissue from 15 day old mice 4 hours post MycER activation compared to wild type controls. RNA-seq of heart tissues from adult mice that have expressed oncogenic H-Ras for 4 weeks followed by MycER activation for 4 hours compared to MycER negative controls.

2019-08-07 | E-MTAB-7595 | ExpressionAtlas

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