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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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E. coli MG1655 pJV300 stationary phase

ABSTRACT: Over-night cultures grown from three single colonies were diluted 1:100 in LB and grown with shaking at 37 °C for 6h. Bacteria were pelleted by centrifugation and fast-freezed in the presence of 0.9 mg/ml lysosyme in TE. Frozen cells were thawed and re-frozen twice in 37 °C and liquid nitrogen, respectively. Total RNA was extracted using TRI-reagent and used for construction of RNA-seq libraries according to the RNAtag-seq method. Libraries were paired-end sequenced using Illumina NextSeq 500 platform.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Liron Argaman

PROVIDER: E-MTAB-9834 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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