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Transcription profiling of C. elegans N2 worms treated with Resveratrol

ABSTRACT: Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM+ resveratrol and allowed to grow till young adult stage (ca 40 hours). Worms were harvested and total RNA extracted using Trizol reagent. Poly-A+ RNA was isolated using Qiagen midi-prep kit and used for microarray hybridization.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Janos Demeter

PROVIDER: E-SMDB-3539 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A role for SIR-2.1 regulation of ER stress response genes in determining C. elegans life span.

Viswanathan Mohan M Kim Stuart K SK Berdichevsky Ala A Guarente Leonard L

Developmental cell 20051101 5

C. elegans SIR-2.1, a member of the Sir-2 family of NAD(+)-dependent protein deacetylases, has been shown to regulate nematode aging via the insulin/IGF pathway transcription factor daf-16. Treatment of C. elegans with the small molecule resveratrol, however, extends life span in a manner fully dependent upon sir-2.1, but independent of daf-16. Microarray analysis of worms treated with resveratrol demonstrates the transcriptional induction of a family of genes encoding prion-like glutamine/aspar ...[more]

PMID: 16256736

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N2 worms treated with Resveratrol

Project description:Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM+ resveratrol and allowed to grow till young adult stage (ca 40 hours). Worms were harvested and total RNA extracted using Trizol reagent. Poly-A+ RNA was isolated using Qiagen midi-prep kit and used for microarray hybridization. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: Resveratrol Keywords: compound_treatment_design

2006-01-25 | GSE4094 | GEO

N2 worms treated with Resveratrol

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Resveratrol treatment of daf-16 mutant C. elegans

Project description:Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM+ resveratrol and allowed to grow till young adult stage (ca 40 hours). Worms were harvested and total RNA extracted using trizol reagent. Poly-A+ RNA was isolated using Qiagen midi-prep kit and used for microarray hybridization. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: Resveratrol Keywords: compound_treatment_design

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Project description:Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM+ resveratrol and allowed to grow till young adult stage (ca 40 hours). Worms were harvested and total RNA extracted using trizol reagent. Poly-A+ RNA was isolated using Qiagen midi-prep kit and used for microarray hybridization. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: Resveratrol Computed

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Transcription profiling of C. elegans strains IG274 wt;frIs7 and CZ5701 dapk-1(ju4);frIs7 infected with Dreshmeria coniosporia

Project description:1.Sample Growth Conditions protocols Eggs from IG274 wt; frIs7 and dapk-1(ju4); frIs7 worms cultivated at 25°C were allowed to hatch in the absence of food at 25°C for 16 hours. Synchronised larvae were transferred to NGM agar plates lawned with OP50 and cultivated at 25°C for 48 hours. 2.Sample Treatment protocols Synchronised IG274 wt; frIs7 and CZ5701 dapk-1(ju4); frIs7 worms at the young L4 stage were infected with freshly harvested spores of Dreshmeria coniosporia. After 24 hours at 25°C, the worms were collected into trizol for further RNA extraction. Controls worms were non infected.

2008-11-28 | E-MEXP-1363 | biostudies-arrayexpress

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Adult Caenorhabditis elegans: wild-type (N2) and mir-243 mutant worms

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2011-09-15 | E-GEOD-20558 | biostudies-arrayexpress

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RNA-seq of N2, HBR2340 and PHX2193 worms in L1 arrest (48 hours)

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Project description:Transcriptional profiling of N2 vs. mir-243 worms, aiming to identify direct and indirect targets of the microRNA.

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