Transcription profiling of C. elegans IG274 wt frIs7 and IG339 nipi-1(fr1) frIs7 after infection with Dreshmeria coniosporia

ABSTRACT: 1.Sample Growth Conditions protocols
Eggs from IG274 wt; frIs7 and IG339 nipi-1(fr1); frIs7 worms cultivated at 25°C were allowed to hatch in the absence of food at 25°C for 16 hours. Synchronised larvae were transferred to NGM agar plates lawned with OP50 and cultivated at 25°C for 48 hours.
2.Sample Treatment protocols
Synchronised IG274 wt; frIs7 and IG339 nipi-1(fr1); frIs7 worms at the young L4 stage were infected with freshly harvested spores of Dreshmeria coniosporia. After 24 hours at 25°C, the worms were collected into trizol for further RNA extraction. Controls worms were non infected.

INSTRUMENT(S): ScanArray 4000XL [PerkinElmer]

ORGANISM(S): Caenorhabditis elegans

DISEASE(S): not infected

SUBMITTER: Jonathan Ewbank 

PROVIDER: E-MEXP-1364 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress