Transcription profiling of C. elegans lin-14 mutants

ABSTRACT: A temporal gradient of the novel nuclear protein LIN-14 specifies the timing and sequence of stage-specific developmental events in Caenorhabditis elegans. The profound effects of lin-14 mutations on worm development suggest that LIN-14 directly or indirectly regulates stage-specific gene expression. We show that LIN-14 can associate with chromatin in vivo and has in vitro DNA binding activity. A bacterially expressed C-terminal domain of LIN-14 was used to select DNA sequences that contain a putative consensus binding site from a pool of randomized double-stranded oligonucleotides. To identify candidates for genes directly regulated by lin-14, we employed DNA microarray hybridization to compare the mRNA abundance of C. elegans genes in wild-type animals to that in mutants with reduced or

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-3919 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress