Transcription profiling of Streptomyces coelicolor to investigate the role of sigma factors

ABSTRACT: The differentiating bacterium Streptomyces coelicolor harbours some 66 sigma factors, which support its complex life cycle. σB, a functional homologue of σS from Escherichia coli, controls both osmoprotection and differentiation in S. coelicolor A3(2). Microarray analysis revealed σB-dependent induction of more than 280 genes by 0.2 M KCl. These genes encode several sigma factors, oxidative defence proteins, chaperones, systems to provide osmolytes, cysteine, mycothiol, and gas vesicle. σB controlled induction of itself and its two paralogues (σL and σM) in a hierarchical order of σB→σL→σM, as revealed by S1 mapping and Western blot analyses. The phenotype of each sigma mutant suggested a sequential action in morphological differenti

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-4094 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress