Transcription profiling of mRNAs that remained associated with polysomes isolated from human cells after infection with poliovirus

ABSTRACT: Although most eukaryotic mRNAs need a functional cap binding complex eIF4F for efficient 5' end- dependent scanning to initiate translation, picornaviral, hepatitis C viral, and a few cellular RNAs have been shown to be translated by internal ribosome entry, a mechanism that can operate in the presence of low levels of functional eIF4F. To identify cellular mRNAs that can be translated when eIF4F is depleted or in low abundance and that, therefore, may contain internal ribosome entry sites, mRNAs that remained associated with polysomes were isolated from human cells after infection with poliovirus and were identified by using a cDNA microarray. Approximately 200 of the 7000 mRNAs analyzed remained associated with polysomes under these conditions. Among the gene products encoded by these po

ORGANISM(S): Homo sapiens

SUBMITTER: Michael B. Eisen 

PROVIDER: E-SMDB-59 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress