Genomics

Dataset Information

46

Transcriptional and polysomal profile of poliovirus-infected HUVECs


ABSTRACT: Translation initiation of eukaryotic mRNAs mostly occurs by the cap-dependent ribosome scanning mechanism. However, certain mRNAs are translated by ribosome assembly at internal ribosome entry sites (IRES), a mechanism that allows the synthesis of certain proteins when cap-dependent translation is inhibited by cellular stress. Whether IRES-mediated translation occurs in stressed human endothelial cells (EC) is unknown. We performed whole genome microarray analysis of polyribosomal mRNA (43,203 sequences) from virus-infected EC to identify IRES-containing mRNAs. Overall design: RNA from polysome fractions or total RNA were isolated from control or PV-infected HUVECs using Trizol LS (Invitrogen, Carlsbad, CA) and then subjected to the whole human genome-wide microarray analysis (43,203 transcripts, Agilent, Santa Clara, CA) to obtain the translational profile.

INSTRUMENT(S): Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Feature Number version)

ORGANISM(S): Homo Sapiens

SUBMITTER: Douglas I. Schmid  

PROVIDER: GSE15356 | GEO | 2009-03-27

SECONDARY ACCESSION(S): PRJNA115773

REPOSITORIES: GEO

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