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Transcription profiling of fission yeast meiosis and sporulation

ABSTRACT: pat1 meiosis is very synchronous (see E-SNGR-2), but the use of a temperature shift and the prolonged nitrogen starvation can lead to artifacts. As a control for the E-SNGR-2 experiment, non-sporulating pat1+ diploids were treated exactly as in the protocol for E-SNGR-2 cells. Under these conditions the cells re-enter the mitotic cycle. As a reference we used a pool consisting of equal amount of RNA from all time points of the experiments. This experiment is part of a series, E-SNGR-2 to E-SNGR-7.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Cordelia Langford

PROVIDER: E-SNGR-5 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The transcriptional program of meiosis and sporulation in fission yeast.

Mata Juan J Lyne Rachel R Burns Gavin G Bähler Jürg J

Nature genetics 20020805 1

Sexual reproduction requires meiosis to produce haploid gametes, which in turn can fuse to regenerate a diploid organism. We have studied the transcriptional program that drives this developmental process in Schizosaccharomyces pombe using DNA microarrays. Here we show that hundreds of genes are regulated in successive waves of transcription that correlate with major biological events of meiosis and sporulation. Each wave is associated with specific promoter motifs. Clusters of neighboring genes ...[more]

PMID: 12161753

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Transcription profiling of fission yeast meiosis and sporulation

Project description:pat1 meiosis is very synchronous (see E-SNGR-2), but the use of a temperature shift and the prolonged nitrogen starvation can lead to artifacts. As a control for the pat1 experiment,non-sporulating pat1+ diploids were treated exactly as in the protocol for pat1 cells, with the exception that no nitrogen was added at the time of the temperature shift. Under these conditions the cells remain blocked in G1. As a reference we used a pool consisting of equal amount of RNA from all time points of the experiments. This experiment is part of a series, E-SNGR-2 to E-SNGR-7.

2002-08-05 | E-SNGR-6 | biostudies-arrayexpress

Transcription profiling of fission yeast meiosis and sporulation

Project description:Transcriptional profile of fission yeast meiosis and sporulation. Fission yeast cells undergo meiosis and sporulation under conditions of nutritional stress, most frequently nitrogen starvation. This is a complex developmental process, which results in the formation of four spores that are highly resistant to environmental stress. We have carried out time courses of diploid fission yeast cells undergoing meiosis and sporulation. To achieve the best possible synchrony we have used thermosensitive mutants in the meiotic inhibitor pat1. We first pre-synchronized the cells in G1 by removal of nitrogen at the permissive temperature for pat1, and then induced meiosis by inactivating pat1 with a temperature shift. pat1-induced meiosis is a standard technique, resulting in a highly synchronous meiosis that is very similar to the normal process. The use of pat1 mutants and a temperature-shift may cause some artifacts, so these results should be viewed together with temperature shift controls and especially with the results of the time course done with wild type diploids, available under accession numbers E-SNGR-3 to E-SNGR-7. In all cases we used as reference a pool consisting of equal amounts of RNA from all time points of the experiment. This experiment describes the first biological replicate for pat1 using array design A-SNGR-2. Note that there are three other biological replicates, which use array design A-SNGR-1 in experiment E-SNGR-2.

2002-08-05 | E-SNGR-7 | biostudies-arrayexpress

Transcription profiling of fission yeast meiosis and sporulation

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