Transcription profiling by array of mouse wild type, or mutant or knocked out for Myb, p300 or Mpl bone marrow lineage- Sca-1+ c-Kit+ cells

ABSTRACT: Thrombopoietin (TPO), acting through its receptor Mpl, has two major physiological roles: ensuring production of sufficient platelets via stimulation of megakaryocyte production, and maintaining hematopoietic stem cell (HSC) quiescence. Mpl also controls circulating TPO concentration via receptor-mediated internalisation and degradation. We demonstrate that the megakaryocytosis and increased platelet mass in mice with mutations in the Myb or p300 genes causes reduced circulating TPO concentration and TPO starvation of the stem cell compartment, which is exacerbated because these cells additionally exhibit impaired responsiveness to TPO. Total RNA obtained from bone marrow Lineage- Sca-1+ c-Kit+ cells of mice that were mutant or knocked out for Myb, p300 or Mpl were compared to wild type s

ORGANISM(S): Mus musculus

SUBMITTER: Carolyn de Graaf 

PROVIDER: E-TABM-1050 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress