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Translation profiling of human lung fibroblasts treated or not with rapamycin to better understand the molecular mechanism behind alternative translational routes

ABSTRACT: Deregulation of translational control is frequently implicated in the establishment and maintenance of the cancer phenotype. Anti-cancer drugs targeting protein synthesis are confronted by the problem that genes can exploit alternative translational mechanisms. To better understand the molecular mechanism behind these alternative translational routes we have exploited an ex-vivo assay that follows a competitive re-recruitment of cellular mRNAs onto polysomes in cells treated or not with rapamycin.

ORGANISM(S): Homo sapiens

SUBMITTER: Joseph Curran

PROVIDER: E-TABM-205 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An approach to analyse the specific impact of rapamycin on mRNA-ribosome association.

Genolet Raphael R Araud Tanguy T Maillard Laetitia L Jaquier-Gubler Pascale P Curran Joseph J

BMC medical genomics 20080801

<h4>Background</h4>Recent work, using both cell culture model systems and tumour derived cell lines, suggests that the differential recruitment into polysomes of mRNA populations may be sufficient to initiate and maintain tumour formation. Consequently, a major effort is underway to use high density microarray profiles to establish molecular fingerprints for cells exposed to defined drug regimes. The aim of these pharmacogenomic approaches is to provide new information on how drugs can impact on ...[more]

PMID: 18673536

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