Transcription profiling of human Hela cells infected with Vaccinia mutants

ABSTRACT: Samples were received from the lab of Bertram Jacobs, experiments done by Jeff Langland ([e-mail]). Midlog Hela cells were infected with copenhagen strain Vaccinia Virus at MOI of 5. Viral stocks were sucrose pad purified. Incubated 2, 6, and 9 hrs post infection. viral mutants include VC2 (WT), VVdeltaE3L (e3L gene deleted), VVdelta83N(83N terminal amino acids of E3L delted, protein wont bind Z-DNA), VVdelta26C (26C terminal amino acids of E3L deleted, Protein wont bind dsRNA), VVdeltaE3l-ATV(E3L replaced with eIF2alpha homolog encoded by the salamander ATV virus. Product inhibits PKR but doesnt block IFN induction),and UV (UV inactivated WT)

ORGANISM(S): Homo sapiens

SUBMITTER: Matthew Thomas 

PROVIDER: E-TABM-64 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress