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Transcription profiling of human Hela cells infected with Vaccinia mutants

ABSTRACT: Samples were received from the lab of Bertram Jacobs, experiments done by Jeff Langland (langland@asu.edu). Midlog Hela cells were infected with copenhagen strain Vaccinia Virus at MOI of 5. Viral stocks were sucrose pad purified. Incubated 2, 6, and 9 hrs post infection. viral mutants include VC2 (WT), VVdeltaE3L (e3L gene deleted), VVdelta83N(83N terminal amino acids of E3L delted, protein wont bind Z-DNA), VVdelta26C (26C terminal amino acids of E3L deleted, Protein wont bind dsRNA), VVdeltaE3l-ATV(E3L replaced with eIF2alpha homolog encoded by the salamander ATV virus. Product inhibits PKR but doesnt block IFN induction),and UV (UV inactivated WT)

ORGANISM(S): Homo sapiens

SUBMITTER: Matthew Thomas

PROVIDER: E-TABM-64 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Suppression of proinflammatory signal transduction and gene expression by the dual nucleic acid binding domains of the vaccinia virus E3L proteins.

Langland Jeffrey O JO Kash John C JC Carter Victoria V Thomas Matthew J MJ Katze Michael G MG Jacobs Bertram L BL

Journal of virology 20061001 20

Cells have evolved elaborate mechanisms to counteract the onslaught of viral infections. To activate these defenses, the viral threat must be recognized. Danger signals, or pathogen-associated molecular patterns, that are induced by pathogens include double-stranded RNA (dsRNA), viral single-stranded RNA, glycolipids, and CpG DNA. Understanding the signal transduction pathways activated and host gene expression induced by these danger signals is vital to understanding virus-host interactions. Th ...[more]

PMID: 17005686

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