RNA-seq of Escherichia coli MG1655 WT strain against strain deleted of the ettA gene and complemented strain
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ABSTRACT: Comparison of transcripts of the WT strain versus the strain deleted of the ettA gene in condition where the deleted strain show a difference in growth rate compared to the WT strain. We also include a complemented strain where the gene ettA was inserted at the P21 locus in the etta deleted strain. Strains were cultivated in MJ9 medium with amino acids as carbon sources and with 0.4 M of NaCl.
Project description:Gene AFR1 was deleted from Cryptococcus neoformans lab strain H99. Approximately 1 million cells of the deletion strain were spread on YPD-agar plate supplemented with 0.125ug/mL uniconazole. Randomly 30 adaptors were chosen. 16 adaptors were resistant to uniconazole and were sequenced.
Project description:The transcriptome of embryos which overexpress Pmar1 is compared to the transcriptomes of embryos which do not express Pmar1. For this purpose, sea urchin embryo RNAs were prepared from 3 experimental conditions: over expression of Pmar, over expression of dominant negative cadherin (dnCad), non injected embryos (NI)
Project description:This SuperSeries is composed of the following subset Series: GSE36224: Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain GSE36225: Comparison of transcript abundance in ungerminated spores of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain Refer to individual Series
Project description:To characterize the molecular basis of cytotoxicity of different Pseudomonas species and strains, we analyzed the protein content of secretomes of three P. chlororaphis strains (CIP63, CIP75 and the reference strain PA23) and of six P. entomophila strains (L48 WT, or deleted for various virulence factors: the global activator GacA, the pore-forming toxin Mnl, the pore-forming toxin ExlA, and double mutants for these genes).
Project description:The lexA gene was deleted from WP3 genome to construct the WP3ΔlexA strain. The two strains were cultured at 20MPa and 4°C and the transcriptional profiles were compared.
Project description:The hns gene was deleted from WP3 genome to construct the WP3Δhns strain. The two strains were cultured at 20°C and the transcriptional profiles were compared.
Project description:The hns gene was deleted from WP3 genome to construct the WP3Δhns strain. The two strains were cultured at 4°C and the transcriptional profiles were compared.
Project description:CEH-60 binding profile by comparison of DNA methylation of a C. elegans strain expression ceh-60::dam to a control strain expressing gfp::dam. Sequencing libraries prepared using NEBNext Singleplex oligos for Illumina®. Data analysis performed with GeneDamIDseq (Sharma, Ritler, and Meister 2016).
Project description:The DNA binding domain of crp gene was deleted from WP3 genome to construct the WP3ΔcrpD2 strain. The two strains were cultured at 20°C and the transcriptional profiles were compared.
Project description:The cAMP binding domain of crp gene was deleted from WP3 genome to construct the WP3ΔcrpD1 strain. The two strains were cultured at 20°C and the transcriptional profiles were compared.