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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human KNS42 cells treated with the dual pI3-kinas/mTOR inhibitor PI-103

ABSTRACT: Sensitivity to temozolomide (TMZ) is restricted to a subset of glioblastoma patients, with the major determinant of resistance a lack of promoter methylation of the gene encoding the DNA methyltransferase MGMT, although other mechanisms are thought to be active. In a genome-wide screen of paediatric and adult glioma cells, we identified a co-ordinated upregulation of HOX gene expression in the MGMT-independent cell line KNS42. As a recent study has proposed a mechanism for this observation whereby transcriptional activation of the HOXA cluster is reversible by a PI3-kinase inhibitor through an epigenetic mechanism involving histone H3K27 trimethylation, we sought to investigate whether this was active in our system. We thus treated KNS42 cells for 24 hours with the dual PI3-kinase / mTOR inhibitor PI-103 at 5x IC50 and carried out gene expression profiling using Illumina HT-12 microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Anita Grigoriadis

PROVIDER: E-TABM-890 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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