Transcription profiling of year-long 10 degree C reduction in water temperature on global gene expression in tail skeletal muscle from adult, male zebrafish

ABSTRACT: The effect of a year-long 10 reduction in water temperature on global gene expression in tail skeletal muscle from adult, male zebrafish was determined using a long oligonucleotide probe set (16,399 65mers from Compugen) spotted onto glass slides. Outbred male zebrafish were obtained from a commercial supplier (Liles Tropical Fish, FL) at 6 months of age. Fish were maintained at 28 until 10 months of age. (see Gerhard et al., Exp Gerontol 37,1055-68, 2002) For temperature reduction, water temperature was decreased by 2.5 per week for 4 weeks. Fish were maintained until 22 months of age. Fish were fed fish flakes (Wardely Corp. Secaucus, NJ) twice per day. Each feeding was a discrete event in which a small measured amount of granular food was sprinkled on the water surface. A second small

ORGANISM(S): Danio rerio

SUBMITTER: Hedieh Sajadi 

PROVIDER: E-TIGR-19 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress