Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse macrophage response to hypoxia, part 2


ABSTRACT: We hypothesize that the culture media collected from macrophages exposed to intermittent hypoxia will induce a greater pro-inflammatory gene profile in naïve cultured macrophages than will culture media collected from macrophages exposed to sustained hypoxia. We will evaluate gene expression using microarray analysis of RNA collected from RAW 264.7 macrophages cultured for 24 hours in DMEM media obtained from 1) cells cultured with intermittent hypoxia (2 minute cycles: 90 seconds at 40 Torr and 30 seconds at 8 Torr), 2) media exposed to intermittent hypoxia, 3) cells cultured with sustained hypoxia (8 Torr), 4) media exposed to sustained hypoxia and 4) standard tissue culture conditions (fresh DMEM media; reference).

ORGANISM(S): Mus musculus

SUBMITTER: Renee Rubio 

PROVIDER: E-TIGR-91 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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