Proteotranscriptomic Discrimination of Tumor and Normal Tissues in Renal Cell Carcinoma.

ABSTRACT: Clear cell renal carcinoma is the most frequent type of kidney cancer, with an increasing incidence rate worldwide. In this research, we used a proteotranscriptomic approach to differentiate normal and tumor tissues in clear cell renal cell carcinoma (ccRCC). Using transcriptomic data of patients with malignant and paired normal tissue samples from gene array cohorts, we identified the top genes over-expressed in ccRCC. We collected surgically resected ccRCC specimens to further investigate the transcriptomic results on the proteome level. The differential protein abundance was evaluated using targeted mass spectrometry (MS). We assembled a database of 558 renal tissue samples from NCBI GEO and used these to uncover the top genes with higher expression in ccRCC. For protein level analysis

SUBMITTER: Bartha A 

PROVIDER: S-EPMC10003397 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature