High-efficiency pharmacogenetic ablation of oligodendrocyte progenitor cells in the adult mouse CNS.

ABSTRACT: Approaches to investigate adult oligodendrocyte progenitor cells (OPCs) by targeted cell ablation in the rodent CNS have limitations in the extent and duration of OPC depletion. We have developed a pharmacogenetic approach for conditional OPC ablation, eliminating >98% of OPCs throughout the brain. By combining recombinase-based transgenic and viral strategies for targeting OPCs and ventricular-subventricular zone (V-SVZ)-derived neural precursor cells (NPCs), we found that new PDGFRA-expressing cells born in the V-SVZ repopulated the OPC-deficient brain starting 12 days after OPC ablation. Our data reveal that OPC depletion induces V-SVZ-derived NPCs to generate vast numbers of PDGFRA⁺NG2⁺ cells with the capacity to proliferate and migrate extensively throughout the

SUBMITTER: Xing YL 

PROVIDER: S-EPMC10014347 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature