The cyclic peptide G4CP2 enables the modulation of galactose metabolism in yeast by interfering with GAL4 transcriptional activity.
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ABSTRACT:
SUBMITTER: Rosa S
PROVIDER: S-EPMC10014601 | biostudies-literature | 2023
REPOSITORIES: biostudies-literature
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The cyclic peptide G4CP2 enables the modulation of galactose metabolism in yeast by interfering with GAL4 transcriptional activity.
Rosa Stefano S Tagliani Andrea A Bertaso Chiara C Tadini Luca L Visentin Cristina C Gourlay Louise Jane LJ Pricl Sabrina S Feni Lucia L Pellegrino Sara S Pesaresi Paolo P Masiero Simona S
Frontiers in molecular biosciences 20230301
Genetically-encoded combinatorial peptide libraries are convenient tools to identify peptides to be used as therapeutics, antimicrobials and functional synthetic biology modules. Here, we report the identification and characterization of a cyclic peptide, G4CP2, that interferes with the GAL4 protein, a transcription factor responsible for the activation of galactose catabolism in yeast and widely exploited in molecular biology. G4CP2 was identified by screening CYCLIC, a Yeast Two-Hybrid-based c ...[more]
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