Inflammasome activation and formation of ASC specks in patients with juvenile idiopathic arthritis.

ABSTRACT:

Objective

The formation of large intracellular protein aggregates of the inflammasome adaptor ASC is a hallmark of inflammasome activation and characteristic of autoinflammation. Inflammasome activated cells release the highly proinflammatory cytokine IL-1β in addition to ASC specks into the extracellular space. Autoinflammatory activity has been demonstrated in systemic JIA, however minimal data exist on the role of inflammasomes in other JIA subtypes. We therefore investigated, if pyroptotic cells are present in the circulation of oligo- and poly-articular JIA.

Methods

Peripheral blood of JIA patients (n = 46) was investigated for ASC speck formation, a key step in inflammasome activation, by flow cytometry and immunofluorescence. Free ASC and proinflammatory cytoki

SUBMITTER: Wittmann N 

PROVIDER: S-EPMC10014801 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature