Optimization of nutrient utilization efficiency and productivity for algal cultures under light and dark cycles using genome-scale model process control.

ABSTRACT: Algal cultivations are strongly influenced by light and dark cycles. In this study, genome-scale metabolic models were applied to optimize nutrient supply during alternating light and dark cycles of Chlorella vulgaris. This approach lowered the glucose requirement by 75% and nitrate requirement by 23%, respectively, while maintaining high final biomass densities that were more than 80% of glucose-fed heterotrophic culture. Furthermore, by strictly controlling glucose feeding during the alternating cycles based on model-input, yields of biomass, lutein, and fatty acids per gram of glucose were more than threefold higher with cycling compared to heterotrophic cultivation. Next, the model was incorporated into open-loop and closed-loop control systems and compared with traditional fed-batch s

SUBMITTER: Li CT 

PROVIDER: S-EPMC10017758 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature